The Human Reference Interactome and Literature Benchmark
At the Center for Cancer Systems Biology at Dana-Farber Cancer Institute all pairwise combinations of human protein-coding genes are systematically being interrogated to identify which are involved in binary protein-protein interactions. In our most recent effort 17,500 proteins have been tested and a first human reference interactome (HuRI) map has been generated. The entirety of all interactions identified in systematic screens at CCSB forms HI-union.
This project is published in Nature.www.nature.com/articles/s41586-020-2188-x
Pairwise combinations of human protein-coding genes are tested systematically using high throughput yeast two-hybrid screens to detect protein-protein interactions. The quality of these interactions is further validated in multiple orthogonal assays. Currently 64006 PPIs involving 9094 proteins have been identified using this framework.
In addition to systematically identifying PPIs experimentally, this web portal also includes PPIs of comparable high quality extracted from literature. This subset of literature-curated PPIs currently comprises 13441 PPIs involving 6047 proteins.